What is the role of embryologist :-
An embryologist is the fertility spacelist that helps to create viable embryos to eighter be used in ivf or frozen for latter use. Embryologist are highly trained medical professional usually holding a master degree or PHD due to nature of work. They are responsible for the careful maintenance and management of genetic material use in creating embryos the collected sperm and egg a well as maintaining these embryos they devlop
Platelets Rich Plasma (P R P);
The research was carried out to asses the effectiveness of PRP in the therapy of infertile woman with a thin endometrium (<7mm)told the ivf doctors.When a subject is undergoing in vitro fertilization with poor endometrium response still have the endometrium which is less then seven mil limiters after standerd hormone replacement therapy (HRT) and had to cancelled the embryo-transfer cycle.Then intra uterine infusion of PRP was performed. PRP was well prepared from autologous blood through centrifugation and 0.5-1 ml of PRP was injected into the uterine cavity in the tenth day of HRT cycle. If endometrial thickness failed to increase 72 hours later. PRP infusion was done 1-2 time in each cycle, Euspension and pregnency were observed in the patient after Prp infusion.
Intrauterine PRP infusion represent a new method for thin endometrium with poor responses. It was reported a platelets rich plasma was ablw to promote the endometrium growth and improve pregnancy out come a patients with a their endometrium
TECHNIQUES OF IUI
There are followings techniques used in iui
Basic sperm wash
Premium sperm wash
Swime up technique
Basic Sperm Wash
This techniques used dilution and centrifugation. A sperm wash solution containing antibiotics and protein supplement is added to the ejaculate. After repeated centrifugation, the seminal fluid id eliminated from the sample and sperm cells are concentrated for insemination. The procedure takes 20 to 40 minute
Premium wash
This method used density gradient centrifugation to isolate the one one prig the mobile sperm in ordar to obtain a sperm sample with the mobility at least 90%of bending upon the initial quantity of sample different concentration of isolate extremely dense five are layer in a test tube in an assending ordar of density having layer of the bottom when a semen sample is placed upon the wroer most isolate layer and centrifuged any debris required cell non motile and poor motile sperm remain in the top layer only mobile sperm are able to get through the bottom layer and are then concentrated for use in artificial insemination. This procedure take one hour. The premium wash technique is excellent for fresh or frozen sperm and can help asses male factor fertility
swime up technique
This technique uses sperm self migration
Intra uterine insemination (IUI)
IUI is a simple procedure that outs sperm directly inside your uterus which helps healthy sperm get closer to your egg
Sperm Wash
Sperm wash is a form of sperm preparation
Sperm washing involves remaining the seminal plasma and any procurement if use the specimen prior to intra uterine insemination
The folloeings techniques use to sperm wash
Basic Sperm wash
Premium Sperm Wash
swimeup technique
HUMAN SPERM IS ABOUT 70 micrometre long
HEAD SIZE 4-5 micrometre
NUCLEUS CONTAIN 23 CHROMOSOME
Acrosome
Mid piece 4-5 micrometre Tail 5.5 micrometre
Mortality propagated along the tail
How many sperm there are (count)
A normal sperm count is at least 15 million sperm perm per millilitre of semen your sperm count is considerd low of you have less then that
How of your sperm are moving (Motility)
your doctor will look at how many sperm are moving and how well they move. Ideally 50%move of your sperm are active
What your sperm look like (Morphology)
The size and shape of your sperm affect their ability to fertilize n egg. Normally semen will have at least 4% normal shaped sperm in addition to analyze your sperm your sperm doctor will alson final out their details from your sample including the sperm
VOLUME
He will note hoe much semen you were able to provide for your sample. A normal ampount is at least 1.5 millilitres about half of tea spoon if your sample is less then that your semen vesicle are not making enough fluid or are blocked. You could also have problem with your prostrate
In order to test your semen your doctor will ask to provide a semen a sample, Normally you ejaculate a semen sample into a collection cup in a private room at your doctors office. Sometime you can collect your sample at your home though you have to keep it at room temperature and get it to your doctor or lab with in 1 hour some doctor you have to provide a special condom that collect semen during sex. your doctor may ask you not to have sex or masturbation for 2 to 5 days before your test to make sure your sperm count will be as high as possible however do not avoid ejaculation for more then 2 weeks before your test. that can result in a sample with sperm that are less active.
It's best not to drink alcohol before your semen analysis. You should also tell your doctor about any medication or herbal supplement you are taking .And don't use lubricant when you collect your sample before they can affect how easily your sperm can move around.
To get your more accurate result your doctor will want to test more then one sample from the same man can vary you may need to provide 2 ton 3 sample over a 3 months period
WHAT THE TEST TELLS YOU
once a lab get your semen sample they will look at it under microscope. This will give them a wealth of information including.
Andrology
Andrology is the medical specialty that deals with the male health problem. Particularly to the male reproductive system and urological problems and with the help of andrology also find male infertility
There are three types of andrology
1) semen analysis
2) plasma rich platelets (PRP)
3) Intrauterine insemination (I U I)
Semen Analysis
If you and your partner having trouble getting pregnant it can be frustrating and even hurt feeling.No doubt you want to find the cause behind whats preventing you from conceiving.
While both man and woman can have problem issue with male fertilization as half of all infertility cause.
And since male infertility is often cause by law sperm production one of the first test of your doctor is likely ask to semen analysis
PROVIDING A SEMEN SAMPLE
